A 3-D High-Frequency Array Based 16 Channel Photoacoustic Microscopy System for <emphasis emphasistype="italic">In Vivo</emphasis> Micro-Vascular Imaging

A 3-D High-Frequency Array Based 16 Channel Photoacoustic Microscopy System for In Vivo Micro-Vascular Imaging

This paper discusses the design of a novel photoacoustic microscopy imaging system with promise for studying the structure of tissue microvasculature for applications in visualizing angiogenesis. A new 16 channel analog and digital high-frequency array based photoacoustic microscopy system (PAM) was developed using an Nd:YLF pumped tunable dye laser, a 30 MHz piezo composite linear array transd...

Photoacoustic Microscopy and Photoacoustic Computed Tomography Using High-frequency Linear Array Ultrasonic Transducers

Photoacoustic imaging of the microvasculature with a high-frequency ultrasound array transducer.

Visualization of microvascular networks could provide new information about function and disease. We demonstrate the capabilities of a 30-MHz ultrasound array system for photoacoustic microscopy of small (< or = 300 microm) vessels in a rat. 3D images obtained by translating the array in the elevation direction are compared with photographs of excised skin. The system is shown to have 100-micro...

Curved array photoacoustic tomographic system for small animal imaging.

We present systematic characterization of a photoacoustic imaging system optimized for rapid, high-resolution tomographic imaging of small animals. The system is based on a 128-element ultrasonic transducer array with a 5-MHz center frequency and 80% bandwidth shaped to a quarter circle of 25 mm radius. A 16-channel data-acquisition module and dedicated channel detection electronics enable capt...

High frequency label-free photoacoustic microscopy of single cells☆

Photoacoustic measurements of melanoma cells and red blood cells (RBCs) using ultra-high frequency (UHF) wide-bandwidth transducers are reported. In this detection system, the resolution typically depends on the parameters of the receiving transducer, and not the focus of the laser. A single melanoma cell was imaged with 200, 375 and 1200 MHz transducers. As the frequency increased, the resolut...